Multiplex Reverse Transcription-PCR Screening for EML4-ALK Fusion Transcripts
نویسندگان
چکیده
منابع مشابه
Multiplex reverse transcription-PCR screening for EML4-ALK fusion transcripts.
PURPOSE EML4-ALK is a fusion-type protein tyrosine kinase that is generated by inv(2)(p21p23) in the genome of non-small cell lung cancer (NSCLC). To allow sensitive detection of EML4-ALK fusion transcripts, we have now developed a multiplex reverse transcription-PCR (RT-PCR) system that captures all in-frame fusions between the two genes. EXPERIMENTAL DESIGN Primers were designed to detect a...
متن کاملA prospective PCR-based screening for the EML4-ALK oncogene in non-small cell lung cancer.
PURPOSE EML4-ALK is a lung cancer oncogene, and ALK inhibitors show marked therapeutic efficacy for tumors harboring this fusion gene. It remains unsettled, however, how the fusion gene should be detected in specimens other than formalin-fixed, paraffin-embedded tissue. We here tested whether reverse transcription PCR (RT-PCR)-based detection of EML4-ALK is a sensitive and reliable approach. ...
متن کاملA prospective PCR–based screening for the EML4-ALK oncogene in non-small cell lung cancer Running title: PCR–based screening for EML4-ALK
Division of Functional Genomics, Jichi Medical University, Tochigi 329-0498, Japan; Department of Respiratory Medicine, Toho University Omori Medical Center, Tokyo 143-8541, Japan; Tohoku University Hospital, Miyagi 980-8574, Japan; Miyagi Cancer Center, Miyagi 981-1293, Japan; First Department of Medicine, Hokkaido University School of Medicine, Hokkaido 060-8638, Japan; Asahikawa Medical Cent...
متن کاملImaging, Diagnosis, Prognosis A Prospective PCR-Based Screening for the EML4-ALK Oncogene in Non–Small Cell Lung Cancer
Purpose: EML4-ALK is a lung cancer oncogene, and ALK inhibitors showmarked therapeutic efficacy for tumors harboring this fusion gene. It remains unsettled, however, how the fusion gene should be detected in specimens other than formalin-fixed, paraffin-embedded tissue. We here tested whether reverse transcription PCR (RT-PCR)-based detection of EML4-ALK is a sensitive and reliable approach. Ex...
متن کاملMultiplex fluorescent RT-PCR to quantify leukemic fusion transcripts.
The detection of chimeric transcripts derived from aberrant chromosomal fusion events provides an exceptionally valuable toolfor the diagnosis of leukemia. We have developed a simple, inexpensive, reproducible, and automated method to quantify RT-PCR products. Our approach utilizesfluorescent PCRfor the co-ampification of the specific fusion transcript with an internal control (HPRT). We have a...
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ژورنال
عنوان ژورنال: Clinical Cancer Research
سال: 2008
ISSN: 1078-0432,1557-3265
DOI: 10.1158/1078-0432.ccr-08-1018